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Immunoexpression of stem cell markers SOX-2, NANOG AND OCT4 in ameloblastoma
Karolyny Martins Balbinot1, Felippe José Almeida Loureiro2, Giordanna Pereira Chemelo2
1Laboratory of Pathological Anatomy and Immunohistochemistry, Federal University of Pará, Belém, Pará, Brazil.
Background:
Ameloblastoma (AME) is characterized by a locally invasive growth pattern. In an attempt to justify the aggressiveness of neoplasms, the investigation of the role of stem cells has gained prominence. The SOX-2, NANOG and OCT4 proteins are important stem cell biomarkers.
Methodology:
To verify the expression of these proteins in tissue samples of AME, dentigerous cyst (DC) and dental follicle (DF), immunohistochemistry was performed and indirect immunofluorescence were performed on the human AME (AME-hTERT) cell line.
Results:
Revealed expression of SOX-2, NANOG and OCT4 in the tissue samples and AME-hTERT lineage. Greater immunostaining of the studied proteins was observed in AME compared to DC and DF (p < 0.001).
Conclusions:
The presence of biomarkers indicates a probable role of stem cells in the genesis and progression of AME.
