Graphene Oxide-Carbamoylated Chitosan Hydrogels with Tunable Mechanical Properties for Biological Applications

Chitosan (CH)-based hydrogels have been extensively researched in numerous biological applications, including drug delivery, biosensing, wound healing, and tissue engineering, to name a few. Previously, modified CH hydrogels by carbamoylation, using potassium cyanate (KCNO) as the cross-linker, have shown improvement in viscoelastic properties and biocompatibility. In this study, graphene oxide (GO) nanofillers are added to carbamoylated CH to form a nanocomposite hydrogel and study the influence of CH molecular weight (M_w) and GO loading concentrations on hydrogel properties. The physical properties (swelling, degradation, and porous structure) of the hydrogels can be tuned as required for cell attachment and spreading by varying both the GO concentration and the M_w of CH. Rheological characterization showed an improvement in the mechanical properties (storage modulus, yield stress, and viscosity) of the synthesized CH-GO hydrogels with an increase in the M_w of CH and the GO concentration. Human retinal pigmented epithelial-1 (RPE-1) cells seeded onto the prepared hydrogel scaffolds showed good cell viability, adhesion, and cell spreading, confirming their cytocompatibility, with dependence on both M_w of CH and GO loading.