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Design, Production, and Characterization of Catalytically Active Inclusion Bodies
Gizem Ölçücü1,2, Karl-Erich Jaeger3,4, Ulrich Krauss5,6
1Institute of Molecular Enzyme Technology, Heinrich Heine University Düsseldorf, Forschungszentrum Jülich, Wilhelm-Johnen-Straße, Jülich, Germany. g.olcucu@fz-juelich.de.
Abstract:
Catalytically active inclusion bodies (CatIBs) are promising biologically produced enzyme/protein immobilizates for application in biocatalysis, synthetic chemistry, and biomedicine. CatIB formation is commonly induced by fusion of suitable aggregation-inducing tags to a given target protein. Heterologous production of the fusion protein in turn yields CatIBs. This chapter presents the methodology needed to design, produce, and characterize CatIBs.

