Colorimetric Detection of Metals Using CdS-NPs Synthesized by an Organic Extract of Aspergillus niger

The use of cadmium sulfide nanoparticles (CdS-NPs) synthesized by fungi presents highly stable chemical and optical characteristics; this makes them a promising alternative for development of colorimetric methods for metal detection. Moreover, application of CdS-NPs is challenging due to the biological material used to carry out synthesis and coating is highly diverse; therefore, it is necessary to evaluate if such components are present in the biological material. Thus, the objective of this work was to detect metallic ions in synthetic water samples using CdS-NPs synthesized by the extract of Aspergillus niger. The conditions to produce fungal extracts were determined through a factorial design 2³; additionally, biomolecules involved in metallic ions detection, synthesis, and coating of CdS-NPs were quantified; the studied biomolecules are NADH, sulfhydryl groups, proteins, and ferric reducing antioxidants (FRAP). CdS-NPs synthesized in this study were characterized by spectrophotometry, zeta potential, and high-resolution transmission electron microscopy (HRTEM). Finally, detection capacity of metallic ions in synthetic water samples was evaluated. It was proved that the methanolic extract of Aspergillus niger obtained under established conditions has the necessary components for both synthesis and coating of CdS-NPs, as well as detection of metallic ions because it was possible to synthesize CdS-NPs with a hexagonal crystalline structure with a length of 2.56 ± 0.50 nm which were able to detect Pb²⁺, Cr⁶⁺, and Fe³⁺ at pH 4 and Co²⁺ at pH 8.