Dual Ion-Selective Membrane Deposited Ion-Sensitive Field-Effect Transistor Integrating a Whole Blood Processing...

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Clinical Diagnostics

Background:

• Traditional blood ion testing requires complex procedures and bulky equipment, hindering real-time bedside monitoring.
• Accurate monitoring of blood ions like sodium (Na+) and potassium (K+) is crucial for immune status assessment and disease diagnosis.
• Existing methods are not suitable for continuous, point-of-care applications.

Purpose of the Study:

• To develop a compact, integrated sensor system for rapid, in situ blood ion sensing.
• To enable on-chip serum extraction from whole blood samples.
• To achieve simultaneous detection of Na+ and K+ ions for improved clinical diagnostics.

Main Methods:

• Development of a dual ion-selective membrane deposited ion-sensitive field-effect transistor (DISM-ISFET) sensor.
• Integration of a microchamber for automated serum extraction and red blood cell sedimentation.
• Sequential dispensing of standard solutions and testing with clinical whole blood samples.
• Comparison of sensor results with a commercial pocket ion meter.

Main Results:

• The DISM-ISFET system successfully extracted 200 μL of serum from 500 μL of whole blood.
• Simultaneous Na+ and K+ ion sensing was achieved with high sensitivity and selectivity.
• Complete sample processing and measurement were accomplished within 10 minutes on a single chip.
• Clinical sample testing showed comparable results to a commercial ion meter.

Conclusions:

• The developed DISM-ISFET sensor system offers a rapid, user-friendly solution for real-time blood ion monitoring.
• Its ability to perform on-chip serum extraction and simultaneous ion sensing addresses limitations of traditional methods.
• This technology holds significant potential for point-of-care applications in diverse medical settings like hemodialysis, operating rooms, and intensive care units.