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Detecting STR profiles from degrading menstrual blood samples and their use as possible evidence in forensic
Tahani Mohamed Ibrahim Al-Hazani1, Wedad Saeed Al-Qahtani2, Tahani Saeed Abboosh3
1Department of Biology, College of Sciences and Humanities, Prince Sattam Bin Abdulaziz University, P.O. Box: 83, Al-Kharj 11940, Saudi Arabia.
Abstract:
This research explores DNA consistency and attempts to detect STR profiles from the degrading menstrual blood samples (MBS) as reliable forensic evidence. Peripheral (PBS) and MBS of 30 healthy fertile females were taken on the menstrual cycle's second day. They were obtained at different time periods (0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 24, and 48 h) at 25 °C. DNA evaluation was fulfilled to analyze DNA profiles. A considerable elevation in the median concentrations of DNA between 0 and 14-h intervals were documented, whereas decreased extents were registered between 16 and 48 h. Moreover, complete STR profiles (24/24) for DNA were discovered in all the intervals (0, 2, and 48 h). Periods of 0-8 h demonstrated the maximum extents of DNA materials. Full STR were discovered in all the intervals (0, 2, and 48 h). Eventually, MBS can be utilized as forensic evidence.
