Simultaneous fluorescence sensing of vitamin B2 and sulfur ions based on fluorescent copper nanoparticles

In this study, the F-CuNPs were synthesized by a modified liquid-phase chemical reduction method. Throughout the preparation process, anhydrous copper sulfate was used as the copper source, and ascorbic acid in the NaOH solution served as the reducing and protective agent. Förster resonance energy transfer (FRET) may exist between F-CuNPs and vitamin B2 due to the large spectral overlap between the fluorescence emission spectra of F-CuNPs and the UV-vis absorption spectra of vitamin B2. Therefore, the detection of vitamin B2 was designed based on a FRET system between F-CuNPs and vitamin B2. With S^2- into the F-CuNPs&VB2 system, the fluorescence intensity of vitamin B2 was quenched, while the fluorescence intensity of F-CuNPs was almost unchanged. There may be a specific reaction between S^2- and vitamin B2. Therefore, the research system can be further used to detect S^2- based on ratiometric fluorescent probe. The research findings show that the linear range of vitamin B2 was 0.51 nM-34.64 nM with a detection limit of 0.25 nM (S/N = 3), the linear range of S^2- was 0.64 μM-60.00 μM with a detection limit of 0.32 μM (S/N = 3). Furthermore, the simultaneous fluorescent sensing system has high sensitivity and selectivity. Therefore, this system was designed and successfully used to detect the content of vitamin B2 and S^2- in actual samples to find a new effective method to detect analytes.