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Related Experiment Video

Updated: Aug 13, 2025

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Genetically modified cell spheroids for tissue engineering and regenerative medicine.

Zhiwei Jiang1, Yi Xu1, Mengdie Fu1

  • 1Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Hangzhou 310000, China.

Journal of Controlled Release : Official Journal of the Controlled Release Society
|January 19, 2023
PubMed
Summary

Genetically modified cell spheroids are promising for tissue regeneration and disease treatment. These engineered cell structures offer enhanced gene expression for therapeutic applications, with potential for organ replacement.

Keywords:
Cell spheroidsGene modificationRegenerative medicineTissue engineering

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Area of Science:

  • Biotechnology and Biomedical Engineering
  • Cell Biology and Tissue Engineering
  • Gene Therapy and Regenerative Medicine

Background:

  • Cell spheroids provide cell-cell interactions, improving survival and paracrine effects for biomedical applications.
  • They are recognized as effective vehicles for gene delivery, crucial for enhancing gene expression.
  • Applications span tissue engineering, regenerative medicine, and understanding disease pathophysiology.

Purpose of the Study:

  • To review recent advancements in genetically modified spheroid cells.
  • To explain the fabrication, applications, and development of these engineered cell structures.
  • To discuss limitations and future directions in the field of genetically modified cell spheroids.

Main Methods:

  • Gene delivery to cell spheroids using viral and non-viral vectors.
  • Utilizing novel technologies such as CRISPR/Cas9 for exogenous gene integration.
  • Fabrication techniques for creating genetically modified cell spheroids.

Main Results:

  • Genetically modified spheroids demonstrate enhanced specific gene expression for tissue regeneration.
  • These modified spheroids show potential for differentiation into various cell types (bone, cartilage, nerve, etc.) and organoids (liver).
  • Successful pre-clinical trials indicate potential for organ replacement therapies.

Conclusions:

  • Genetically modified cell spheroids represent a significant advancement in regenerative medicine and gene therapy.
  • Their versatility in differentiation and gene delivery capabilities offers broad therapeutic potential.
  • Further research into fabrication, limitations, and future directions is essential for clinical translation.