CO₂ gradient domestication improved high-concentration CO₂ tolerance and photoautotrophic growth of Euglena gracilis

In order to improve CO₂ biofixation efficiency of microalgae cultivated with coal-chemical flue gas, CO₂ gradient domestication was employed to improve high-concentration CO₂ tolerance and photoautotrophic growth of acid-tolerant Euglena gracilis. The dried biomass yield of photoautotrophic growth of E.gracilis increased from 1.09 g/L (wild-type strain) by 21 % to 1.32 g/L with CO₂ gradient domestication to 15 % CO₂. The RuBisCO activity and biomass production of E.gracilis strain domesticated to 99 % CO₂ were 2.63 and 3.4 times higher, respectively, than those of wild-type strain. The chlorophyll a and b contents were 2.52 and 1.79 times higher, respectively, than those of wild-type strain. Superoxide dismutase and catalase activities of 99 % CO₂-domesticated strain increased to 1.24 and 6 times, which reduced peroxide damage under high carbon stress and resulted in lower apoptotic and necrotic rates of domesticated strain. Thus, this work provides valuable guidance for CO₂ fixation and adaptive evolution of E. gracilis in industrial flue gas.