Composite carrier enhanced bacterial adhesion and nitrogen removal in partial nitrification/anammox process

The rapid start-up and stable operation of one-stage (Partial nitrification/anammox) PN/A process for low-ammonium wastewater are difficult to be achieved, and many carriers are designed to solve this problem. Here, a composite carrier was developed, in which sepiolite and non-woven fabrics were assembled in polypropylene spherical shells. At the start-up phase, P_A reactor using the composite carriers reached a higher nitrogen removal rate of 134.50 ± 19.60 mg·N·L^-1d^-1, in contrast to that of 48.85 ± 19.64 mg·N·L^-1d^-1 in the P_B reactor without sepiolite carriers. When the final influent ammonium concentration of PN/A process is 100 mg/L, the total nitrogen removal efficiency can reach 72 ± 0.03 %. High biomass immobilization ability of composite carrier was evidenced by the greater adsorption trend between sludge and sepiolite than that between sludge and non-woven fabrics, where hydrophobic interaction and Van der Waals interaction played a major role. Extracellular protein (PN) content and the ratio of PN and extracellular polysaccharide of samples in P_A were significantly higher than those in P_B, verifying higher biofilm formation ability on the composite carrier. The composite carrier also increased the abundance of dominant bacteria in PN/A process, especially AOB, the relative abundance of which reached 46.11 %. And it increased the abundance of essential functional genes for nitrogen conversion as their perfect acid neutralizing effects. This study is of great significance in improving the start-up speed and stable operation of this process.