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Updated: Aug 13, 2025
Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
Published on: March 21, 2018
Selection and electrochemical-sensor application of an DNA-aptamer for methyl parathion detection
Wenfei Guo1, Changchun Hu1, Shuo Li1
1Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou, 412007, PR China.
Abstract:
An aptamer-based electrochemical sensor for methyl parathion (MP) detection is herein reported. The modified magnetic beads-systematic evolution of ligands by enrichment (MB-SELEX) was used to select the MP aptamer. After 14 rounds of selection, the aptamer (MPapta-6) with high affinity for MP was obtained, and its dissociation constant (Kd) was 39.66 ± 14.73 μM. Using the MPapta-6, the ultra-sensitive electrochemical sensor based on PLL-BP and AuNPs was constructed. The linear range of MP was 1-105 pM and detection limit (LOD) was as low as 0.49 pM. In addition, the application of the sensor in water samples was verified, and the recovery rate was 96.6%-103.5%. The results from this study showed that this strategy could be applied in practical detection.
