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Related Experiment Video

Updated: Aug 13, 2025

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip
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An integrated dual-layer microfluidic platform for multiple respiratory viruses screening.

Hua Wang1, Jingsong Xu1, Shun Li2

  • 1Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.

Analytica Chimica Acta
|January 19, 2023
PubMed
Summary

This study introduces a microfluidic platform for rapid, simultaneous detection of SARS-CoV-2 and influenza viruses. The dual-layer chip (Dμchip) offers high sensitivity and accuracy for multiple virus screening.

Keywords:
CRISPR/Cas12aDual microchipInfluenza virusesSARS-CoV-2

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Area of Science:

  • Biotechnology
  • Molecular Diagnostics
  • Infectious Disease Research

Background:

  • The global spread of COVID-19 necessitates rapid diagnostic tools, especially given its co-occurrence with influenza.
  • Accurate and simultaneous detection of SARS-CoV-2 and influenza viruses is crucial for effective public health management and preventing outbreaks.

Purpose of the Study:

  • To develop an integrated dual-layer microfluidic platform for specific and highly sensitive simultaneous detection of SARS-CoV-2, Influenza A (H1N1, H3N2), and Influenza B.
  • To evaluate the platform's limit of detection, specificity, and performance in clinical samples compared to existing methods.

Main Methods:

  • An integrated dual-layer microfluidic chip (Dμchip) was designed for real-time fluorescence detection.
  • Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) combined with Cas12a cleavage was employed for viral RNA detection.
  • The platform was validated using spiked samples and 75 clinical samples against RT-PCR.

Main Results:

  • The Dμchip assay demonstrated a limit of detection of 10 copies for SARS-CoV-2, FluA (H1N1, H3N2), and FluB RNAs.
  • High specificity was observed, with no cross-reactivity against other coronaviruses.
  • The assay achieved a positive percentage agreement of 97.9% and a negative percentage agreement of 100% compared to RT-PCR.

Conclusions:

  • The developed microfluidic platform provides a rapid, sensitive, and specific method for simultaneous screening of SARS-CoV-2 and influenza viruses.
  • This technology is suitable for various healthcare settings, including emergency, community, and primary care, facilitating remote diagnosis and outbreak control.