Platelet glucose metabolism in type I diabetic subjects
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Updated: Oct 13, 2026

A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination
Published on: June 25, 2014
G Leoncini1, M Maresca, F Balestrero
1Istituto di Chimica Biologica, Universita' di Genova, Italia.
Abstract:
In a group of 35 type I diabetics, platelet glycolytic and glycogenolytic flux have been measured. In type I diabetic platelets glucose uptake was significantly reduced. The quite normal lactate production was obtained by a faster utilization of stored glycogen in these patients. Specific activity of glycolytic enzymes was normal, in particular the levels and kinetic properties of soluble and membrane bound hexokinase. The rate of glucose flux through the hexose monophosphate shunt, measured either in resting or in arachidonic acid-stimulated platelets, was normal in diabetics. Kinetics of glucose transport across plasma membrane have also been determined. Km was significantly increased in diabetic patients. No changes was shown in Vmax. Modifications present in membrane organization of patients could involve glucose transport protein.
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