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Updated: Aug 13, 2025

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization
Published on: June 28, 2012
A chromosome-scale reference genome assembly of the great sand eel, Hyperoplus lanceolatus
Sven Winter1,2,3, Jordi de Raad2,4, Magnus Wolf1,2
1Institute for Ecology, Evolution, and Diversity, Goethe University, Frankfurt am Main, Germany.
Abstract:
Despite increasing sequencing efforts, numerous fish families still lack a reference genome, which complicates genetic research. One such understudied family is the sand lances (Ammodytidae, literally: "sand burrower"), a globally distributed clade of over 30 fish species that tend to avoid tidal currents by burrowing into the sand. Here, we present the first annotated chromosome-level genome assembly of the great sand eel (Hyperoplus lanceolatus). The genome assembly was generated using Oxford Nanopore Technologies long sequencing reads and Illumina short reads for polishing. The final assembly has a total length of 808.5 Mbp, of which 97.1% were anchored into 24 chromosome-scale scaffolds using proximity-ligation scaffolding. It is highly contiguous with a scaffold and contig N50 of 33.7 and 31.3 Mbp, respectively, and has a BUSCO completeness score of 96.9%. The presented genome assembly is a valuable resource for future studies of sand lances, as this family is of great ecological and commercial importance and may also contribute to studies aiming to resolve the suprafamiliar taxonomy of bony fishes.
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