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An early intermediate of refolding alpha-lactalbumin forms within 20 ms
1Institute of Protein Research, Academy of Sciences of the USSR, Moscow Region.
FEBS Letters
|November 2, 1987
Abstract:
The kinetics of alpha-lactalbumin refolding were studied by the stopped-flow method with the registration of CD and intrinsic fluorescence at several wavelengths. It was shown that the early kinetic intermediate forms during the dead-time of the experiment (20 ms). This intermediate has a considerable amount of secondary structure and unpolar clusters in its molecular structure but has no rigid tertiary structure.