Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

COP Coated Vesicles00:59

COP Coated Vesicles

7.9K
Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
7.9K
Coat Assembly and GTPases01:33

Coat Assembly and GTPases

3.6K
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
3.6K
Viral Recombination00:57

Viral Recombination

23.7K
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
23.7K
Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

2.6K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
2.6K
Subviral Agents01:29

Subviral Agents

71
Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
71

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SV40 exploits the Nesprin-2-SUN1-KPNA4 axis for stepwise targeting and entry into the host nucleus to promote infection.

PLoS pathogens·2026
Same author

Cargo-Adaptor Cooperation Programs Retromer Coat Architecture.

bioRxiv : the preprint server for biology·2026
Same author

The nuclear import receptor importin-7 targets HPV from the Golgi to the nucleus to promote infection.

Science advances·2025
Same author

A modular vaccine platform for optimized lipid nanoparticle mRNA immunogenicity.

Nature biomedical engineering·2025
Same author

An ER-associated structure sequesters misassembled FG-rich nucleoporins to help maintain nuclear pore complex function.

Journal of cell science·2025
Same author

γ-secretase facilitates retromer-mediated retrograde transport.

Journal of cell science·2025

Related Experiment Video

Updated: Aug 13, 2025

Merkel Cell Polyomavirus Infection and Detection
13:45

Merkel Cell Polyomavirus Infection and Detection

Published on: February 7, 2019

10.0K

HPV is a cargo for the COPI sorting complex during virus entry.

Mara C Harwood1,2, Tai-Ting Woo1, Yuka Takeo3

  • 1Department of Cell and Developmental Biology, University of Michigan Medical School, 109 Zina Pitcher Place, Ann Arbor, MI 48109, USA.

Science Advances
|January 20, 2023
PubMed
Summary

Human papillomavirus (HPV) uses the coat protein complex I (COPI) to traffic through the Golgi apparatus during cell entry. This interaction is crucial for HPV infection, revealing viruses can hijack cellular transport machinery.

More Related Videos

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy
06:40

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

Published on: November 4, 2018

6.3K
Viral Nanoparticles for In vivo Tumor Imaging
14:04

Viral Nanoparticles for In vivo Tumor Imaging

Published on: November 16, 2012

17.3K

Related Experiment Videos

Last Updated: Aug 13, 2025

Merkel Cell Polyomavirus Infection and Detection
13:45

Merkel Cell Polyomavirus Infection and Detection

Published on: February 7, 2019

10.0K
Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy
06:40

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

Published on: November 4, 2018

6.3K
Viral Nanoparticles for In vivo Tumor Imaging
14:04

Viral Nanoparticles for In vivo Tumor Imaging

Published on: November 16, 2012

17.3K

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Human papillomavirus (HPV) infects cells by entering through the cell surface and trafficking to the nucleus.
  • The precise mechanisms by which HPV navigates the trans-Golgi network (TGN) and Golgi apparatus remain largely unknown.
  • Understanding HPV's intracellular journey is key to developing antiviral strategies.

Purpose of the Study:

  • To identify host factors involved in HPV trafficking through the TGN/Golgi.
  • To elucidate the role of these factors in enabling HPV nuclear entry and infection.
  • To characterize the interaction between HPV and host cellular machinery during entry.

Main Methods:

  • Utilized cellular fractionation and unbiased proteomics to identify interacting proteins.
  • Employed gene knockdown strategies to assess the function of candidate host factors.
  • Analyzed HPV L2 protein interactions with identified host factors using biochemical assays.

Main Results:

  • Identified coat protein complex I (COPI) as a critical host factor for HPV infection.
  • Demonstrated direct binding between the HPV L2 protein's cytoplasmic segment and COPI upon reaching the TGN/Golgi.
  • Showed that COPI depletion leads to HPV accumulation in the TGN/Golgi, impairing infection.

Conclusions:

  • The L2-COPI interaction facilitates HPV trafficking through the TGN and Golgi stacks during viral entry.
  • This study reveals that incoming HPV virions are effectively hijacked as cargo by the COPI complex.
  • This discovery provides new insights into viral hijacking of retrograde transport pathways for infection.