Related Experiment Videos
Polymorphism of unique noncoding DNA sequences in wild and laboratory mice
F Figueroa1, M Kasahara, H Tichy
1Max-Planck-Institut für Biologie, Abteilung Immungenetik, Tübingen, Federal Republic of Germany.
Genetics
|September 1, 1987
Summary
DNA probes reveal distinct genetic patterns between laboratory and wild mice. European wild mice likely did not contribute to the ancestral stocks of inbred laboratory strains.
Area of Science:
- Genetics
- Molecular Biology
- Evolutionary Biology
Background:
- Chromosome 17 in mice harbors crucial genetic complexes, including the major histocompatibility complex (H-2) and t complex genes.
- Understanding the genetic diversity and origins of laboratory mouse strains is vital for accurate research.
- Unique DNA sequences can serve as markers for population genetics studies.
Purpose of the Study:
- To characterize two novel DNA probes, D17Tu1 and D17Tu2, derived from mouse chromosome 17.
- To investigate the genetic diversity and origins of inbred laboratory mouse strains by comparing them with wild mouse populations.
- To explore the evolutionary history and colonization patterns of different mouse species in Europe.
Main Methods:
- Isolation of DNA probes (D17Tu1 and D17Tu2) from a mouse genomic DNA library.
- Mapping of DNA probes to specific regions of mouse chromosome 17, including the centromeric region and the H-2 complex.
- DNA typing of inbred mouse strains and wild mouse populations (Mus musculus and Mus domesticus) to analyze probe patterns.
Main Results:
- D17Tu1 and D17Tu2 probes contain unique, nonrepetitive sequences and map to chromosome 17.
- Four distinct D17Tu1 patterns were identified in inbred mouse strains, with varying frequencies.
- European wild mice exhibited D17Tu1 patterns not found in laboratory mice, suggesting different ancestral origins.
- Mus domesticus populations showed significantly higher D17Tu1 polymorphism compared to Mus musculus.
- D17Tu2 patterns in inbred strains correlated with the distribution of H-2 haplotypes.
Conclusions:
- Inbred laboratory mouse strains were likely not derived from the sampled European wild mouse populations.
- The distinct genetic patterns suggest different colonization histories for Mus musculus and Mus domesticus in Europe.
- The D17Tu2 probe's correlation with H-2 haplotypes provides insights into the genetic structure of the major histocompatibility complex.
- These findings contribute to understanding mouse population genetics and evolutionary relationships.