Effect of granular activated carbon and chloroform on chain elongation with simple substrate ethanol and acetate

Chain elongation is a promising technology for production of medium-chain fatty acids (MCFAs). Granular activated carbon (GAC) is commonly used in anaerobic fermentation. Low level CHCl₃ can inhibit methanogenesis and homoacetogenesis at the same time. However, the effect of them on chain elongation performance with highly enriched consortia and simple substrate (i.e., ethanol and acetate) was still unclear. Hence, the effects of CHCl₃ and on MCFAs production and the microbial community was studied here. CHCl₃ displayed fatal effect on chain elongation system when its concentration was higher than 0.1% v/v. 0.05% v/v CHCl₃ was enough to inhibit homoacetogens and further decreased the caproate production efficiency without altering the core bacteria tremendously. GAC was found to be adverse for chain elongation with simple substrate (i.e., ethanol and acetate) and highly enriched microbial consortia dominated by Clostridium sensu stricto, less than 20% electrons were finally distributed in caproate. It might be attributed to other electron consuming activities induced by GAC.