Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K
RNA-seq03:21

RNA-seq

10.2K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.2K
RNA01:30

RNA

RNARibonucleic acid (RNA) is a molecule that helps cells carry out essential functions, including protein synthesis. RNA plays a crucial role in transferring genetic information from DNA to the parts of the cell responsible for building proteins. Unlike DNA, which is double-stranded, RNA is single-stranded and can move within the cell to perform various tasks. Additionally, RNA contains the nitrogenous base uracil (U) instead of thymine (T), which is found in DNA.Science and Engineering...
RNA Stability01:53

RNA Stability

33.8K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.8K
In-situ Hybridization02:31

In-situ Hybridization

9.5K
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
9.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization and activity enhancement of a novel thermostable 3-quinuclidinone reductase through modulating the microenvironment of catalytic residues.

Bioresource technology·2026
Same author

Multimodal imaging observation of torpedo maculopathy.

GMS ophthalmology cases·2026
Same author

NanoTAC in targeted protein degradation: Intelligent delivery platforms and synergistic therapeutic paradigms.

Advanced drug delivery reviews·2026
Same author

Long-term observations uncover sustained carbon dioxide emissions from lakes following aquaculture retreat.

Water research·2026
Same author

TIMELESS Promotes LUAD Growth via Suppressing Transferrin-Mediated Ferroptosis and Reprograms the Tumor Microenvironment against Anti-PD-1 Immunotherapy.

Cancer communications (London, England)·2026
Same author

The effects of neoadjuvant sintilimab versus pembrolizumab combined with chemotherapy on resectable non-small cell lung cancer: a multicenter propensity score-matched study.

Translational lung cancer research·2026
Same journal

Metabolic control of RNA splicing by polyamines.

Trends in biochemical sciences·2026
Same journal

The role of glycan modifications in health and disease.

Trends in biochemical sciences·2026
Same journal

Strengthening the philosophical basis of graduate science education.

Trends in biochemical sciences·2026
Same journal

CycloPepper learns cyclization sites in therapeutic peptides.

Trends in biochemical sciences·2026
Same journal

Glycosphingolipids in cell identity: Biosynthesis, functions, and emerging tools.

Trends in biochemical sciences·2026
Same journal

Cap in hand: giant viruses, stolen translation, and a road to endosymbiosis?

Trends in biochemical sciences·2026
See all related articles

Related Experiment Video

Updated: Aug 13, 2025

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

4.4K

In vivo single-molecule RNA structural profiling.

Zhenyin Chen1, Hongtao Duan2, Xing Li3

  • 1Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, PR China; University of Chinese Academy of Science, Beijing 100049, PR China.

Trends in Biochemical Sciences
|January 20, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method to study RNA structures in living cells. They discovered that noncoding RNA molecules can adopt multiple shapes, which change with temperature, impacting their function.

Keywords:
flowering timein vivolong noncoding RNAssingle-molecule RNAstructural profilingstructure and function

More Related Videos

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

12.1K
An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

6.7K

Related Experiment Videos

Last Updated: Aug 13, 2025

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

4.4K
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

12.1K
An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

6.7K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Cellular RNAs display significant structural and functional diversity.
  • Understanding RNA structural dynamics is crucial for deciphering cellular processes.

Purpose of the Study:

  • To develop and apply an in vivo single-molecule RNA structure profiling method.
  • To investigate the structural heterogeneity and temperature-dependent conformational changes of noncoding transcripts.

Main Methods:

  • Development of an in vivo single-molecule RNA structure probing technique.
  • Analysis of structural conformations adopted by individual noncoding RNA isoforms.
  • Assessment of structural and abundance changes in response to varying temperatures.

Main Results:

  • Individual noncoding RNA isoforms exist in multiple distinct structural conformations within living cells.
  • These diverse structures are functionally relevant.
  • RNA structure and the abundance of different conformations are sensitive to temperature fluctuations.

Conclusions:

  • The study reveals unprecedented insights into the dynamic structural landscape of noncoding RNAs in vivo.
  • The developed methodology enables the study of RNA structure-function relationships under physiological conditions.
  • Temperature acts as a significant regulator of noncoding RNA structural dynamics and potentially their biological roles.