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Related Concept Videos

Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

68
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
68

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Multidrug-Resistant ESBL-Producing E. coli in Clinical Samples from the UK.

Delveen R Ibrahim1,2, Christine E R Dodd2, Dov J Stekel2,3

  • 1Department of Biology, School of Science, The University of Duhok, Duhok 42001, Iraq.

Antibiotics (Basel, Switzerland)
|January 21, 2023
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Summary

Multidrug-resistant E. coli infections are a growing threat due to resistance genes like blaCTX-M. Understanding these genetic determinants is crucial for developing effective antibiotic therapy guidelines and improving surveillance.

Keywords:
ESBLEscherichia colicephalosporin-resistantmultidrug-resistant

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Area of Science:

  • Microbiology
  • Genetics
  • Infectious Diseases

Background:

  • Cephalosporin therapy failure is a significant global challenge in infection control.
  • Multidrug-resistant (MDR) Escherichia coli (E. coli) producing extended-spectrum β-lactamases (ESBLs) and/or plasmid-encoded AmpC (pAmpC) β-lactamases are key contributors to resistance.
  • Investigating the genetic basis of MDR E. coli is essential for understanding and combating antibiotic resistance.

Purpose of the Study:

  • To determine the prevalence of ESBL/pAmpC genetic determinants in phenotypically MDR E. coli from clinical samples in a UK hospital.
  • To correlate specific resistance genes with observed antibiotic resistance patterns.
  • To inform the development of improved antibiotic stewardship and surveillance strategies.

Main Methods:

  • Phenotypic resistance profiling of 35 MDR E. coli isolates against 18 antibiotics across seven classes.
  • Molecular detection of ESBL, pAmpC, and integrase genes (blaCTX-M, blaTEM, blaOXA-1, Intl1).
  • Sequence typing to identify specific gene subtypes, such as CTX-M-15 and TEM-1.

Main Results:

  • 32 out of 35 isolates (91.4%) were confirmed as MDR, exhibiting resistance to 4-16 antibiotics.
  • blaCTX-M, blaTEM, and blaOXA-1 genes were detected in 23, 13, and 12 isolates, respectively. Intl1 was found in 17 isolates.
  • Significant correlations were observed between specific genes (e.g., blaCTX-M, Intl1) and resistance to various antibiotic classes, including cephalosporins, fluoroquinolones, and trimethoprim/sulfamethoxazole. No pAmpC genes were identified.

Conclusions:

  • A limited set of genes, particularly blaCTX-M, blaTEM, and blaOXA-1, can confer MDR phenotypes in E. coli.
  • The identified resistance patterns underscore the urgent need for enhanced antibiotic therapy guidelines, reduced antibiotic usage, and improved surveillance.
  • Targeted molecular detection of key resistance genes can aid in understanding and managing MDR E. coli infections.