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Mice Placental ECM Components May Provide A Three-Dimensional Placental Microenvironment
Rodrigo da Silva Nunes Barreto1, Ana Claudia Oliveira Carreira1,2, Mônica Duarte da Silva1
1Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo Cidade Universitária, Butantã CEP 05508-270, Brazil.
Bioengineering (Basel, Switzerland)
|January 21, 2023
Summary
This study analyzed mouse placental extracellular matrix (ECM) proteins to develop a 3D model. Researchers identified key ECM components essential for placental structure and function, overcoming limitations in human placental research.
Area of Science:
- Reproductive Biology
- Proteomics
- Biomaterials Science
Background:
- Human placental studies face bioethical limitations, often relying on term placentas or murine models.
- The placental extracellular matrix (ECM) is crucial for materno-fetal interactions and tissue structure.
- Developing biomimetic models is essential for advancing placental physiology research.
Purpose of the Study:
- To investigate the collagenous and non-collagenous components of the term mouse placental ECM.
- To identify key ECM proteins that can be used to create a 3D model mimicking the placental microenvironment.
- To propose a potential 3D model for reconstructing the placental microenvironment.
Main Methods:
- Proteomic analysis of 18.5-day-old control and decellularized mouse placentas using Orbitrap Fusion Lumos.
- Quantification of protein LFQ intensity via MaxQuant software.
- Filtering and enrichment analysis of ECM and cell junction-related proteins.
Main Results:
- Proteomic analysis identified 2317 proteins, with 118 (5.1%) related to ECM and cell junctions.
- No significant differential expression was observed in 76 (64.4%) of these filtered proteins between control and decellularized groups.
- Enriched ontologies included cell junction, collagen, cell adhesion, vasculature, proteolysis, and ECM organization.
Conclusions:
- Preserved ECM proteins contribute to placental tissue stiffness and cell anchoring.
- These findings support the development of a 3D model for placental microenvironment reconstruction.
- The proposed model can help overcome limitations in studying placental physiology.
