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Storable Cell-Laden Alginate Based Bioinks for 3D Biofabrication.

Anastassia Kostenko1,2, Che J Connon1,2, Stephen Swioklo1

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Bioengineering (Basel, Switzerland)
|January 21, 2023
PubMed
Summary

This study introduces a novel method for storing cells in alginate at room temperature for later 3D bioprinting. This advance simplifies bioink preparation and supports on-demand bioprinting for tissue engineering applications.

Keywords:
3D printingalginatebiofabricationbiological preservationmesenchymal stem cell

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Bioprinting Technology

Background:

  • Three dimensional (3D) bioprinting offers significant potential for drug discovery and tissue engineering.
  • Clinical translation of bioprinted constructs requires efficient biologistics, including cell storage and transportation.
  • Alginate is a widely used hydrogel bioink due to its biocompatibility and ease of modification.

Purpose of the Study:

  • To develop a cost-effective system for storing alginate-encapsulated cells at controlled room temperature (CRT).
  • To reformulate stored cells into an extrudable bioink for on-demand 3D bioprinting.
  • To investigate the use of alginate for both cell storage and subsequent bio-printing.

Main Methods:

  • Human adipose-derived stem cells were encapsulated in crosslinked alginate and stored at CRT (15 °C) for one week.
  • Stored alginate-encapsulated cells underwent degelation and mixing with a second alginate solution to create a printable bioink.
  • The printability of the reformulated bioink was optimized using extrusion-based 3D bioprinting.

Main Results:

  • A four-step process enabled CRT storage of alginate-encapsulated cells.
  • Optimal bioink printability was achieved with 4% and 5% (w/v) alginate concentrations.
  • Stored cells showed a high viable cell recovery of 88 ± 18% after one week at 15 °C.
  • Printed constructs exhibited excellent post-print cell viability and distribution.

Conclusions:

  • A simple and adaptable method integrates room temperature storage with on-demand 3D bioprinting.
  • This approach enhances the feasibility of clinical translation for bioprinted tissues.
  • Utilizing alginate for both storage and printing streamlines the bioprinting workflow.