Electrochemical Aptasensor Developed Using Two-Electrode Setup and Three-Electrode Setup: Comprising Their Current...

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Biosensors

Background:

• Dengue virus poses a significant global health threat, necessitating sensitive and accessible diagnostic tools.
• Current diagnostic methods can be complex and require specialized laboratory equipment.
• Paper-based analytical devices offer a low-cost, portable, and user-friendly alternative for point-of-care diagnostics.

Purpose of the Study:

• To fabricate and electrochemically characterize a novel paper-based sensor for dengue virus detection.
• To compare the performance of two-electrode and three-electrode systems for dengue virus analysis.
• To establish a sensitive and efficient platform for disease diagnosis.

Main Methods:

• Fabrication of a paper-based device using screen-printing technology and black carbon conductive ink.
• Electrochemical characterization of two-electrode and three-electrode systems.
• Detection of dengue virus and analysis of current response ranges and amplification.

Main Results:

• Both two-electrode and three-electrode systems successfully detected dengue virus with a limit of detection (LOD) of 0.1 µg/mL.
• The three-electrode system exhibited a substantially higher current range (55.53 µA to 322.21 µA) compared to the two-electrode system (0.85 µA to 4.54 µA).
• The three-electrode system showed approximately 50 times greater current amplification than the two-electrode system.

Conclusions:

• The three-electrode paper-based sensor is a highly sensitive and viable platform for dengue virus detection.
• This technology holds promise for the sensitive diagnosis of dengue and potentially other diseases.
• The developed sensor offers a cost-effective and portable solution for disease diagnostics.