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Lasp1 Expression Is Implicated in Embryonic Development of Zebrafish.

Ilaria Grossi1, Marco Schiavone1, Elena Cannone1

  • 1Department of Molecular and Translational Medicine, Division of Biology and Genetics, University of Brescia, 25123 Brescia, Italy.

Genes
|January 21, 2023
PubMed
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This summary is machine-generated.

This study reveals the crucial role of LIM and SH3 domain protein 1 (LASP1) in embryonic development using zebrafish. LASP1 is essential for normal organogenesis and preventing apoptosis during vertebrate development.

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Background:

  • LIM and SH3 domain protein 1 (LASP1) is known as a cytoskeleton protein overexpressed in cancers.
  • Its role in physiological conditions and embryonic development remains largely unknown.

Purpose of the Study:

  • To investigate the function of Lasp1 during embryonic development in zebrafish.
  • To characterize Lasp1 protein expression patterns in developing zebrafish and adult organs.

Main Methods:

  • Western blotting, 3D light-sheet microscopy, and fluorescent immunohistochemistry were used to detect Lasp1 expression.
  • Zebrafish morpholino (MO) knockdown was employed to study the effects of Lasp1 deficiency.
  • Digital droplet PCR (ddPCR) was used to quantify mRNA levels.
Keywords:
Lasp1 expressionapoptosisembryonic developmentzebrafish model

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Main Results:

  • Lasp1 protein is expressed at various developmental stages and in adult zebrafish organs.
  • Specific lasp1 MO injection caused developmental abnormalities and increased apoptosis.
  • Knockdown of Lasp1 was confirmed, and MO specificity was validated through mRNA rescue experiments.

Conclusions:

  • Lasp1 plays a significant role in zebrafish embryonic development and organogenesis.
  • Zebrafish serve as a suitable model for studying Lasp1 function in developmental processes.
  • Further research into Lasp1's developmental functions is warranted.