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Lasp1 Expression Is Implicated in Embryonic Development of Zebrafish.
Ilaria Grossi1, Marco Schiavone1, Elena Cannone1
1Department of Molecular and Translational Medicine, Division of Biology and Genetics, University of Brescia, 25123 Brescia, Italy.
Genes
|January 21, 2023
Summary
This study reveals the crucial role of LIM and SH3 domain protein 1 (LASP1) in embryonic development using zebrafish. LASP1 is essential for normal organogenesis and preventing apoptosis during vertebrate development.
Area of Science:
- Developmental Biology
- Cell Biology
- Molecular Biology
Background:
- LIM and SH3 domain protein 1 (LASP1) is known as a cytoskeleton protein overexpressed in cancers.
- Its role in physiological conditions and embryonic development remains largely unknown.
Purpose of the Study:
- To investigate the function of Lasp1 during embryonic development in zebrafish.
- To characterize Lasp1 protein expression patterns in developing zebrafish and adult organs.
Main Methods:
- Western blotting, 3D light-sheet microscopy, and fluorescent immunohistochemistry were used to detect Lasp1 expression.
- Zebrafish morpholino (MO) knockdown was employed to study the effects of Lasp1 deficiency.
- Digital droplet PCR (ddPCR) was used to quantify mRNA levels.
Main Results:
- Lasp1 protein is expressed at various developmental stages and in adult zebrafish organs.
- Specific lasp1 MO injection caused developmental abnormalities and increased apoptosis.
- Knockdown of Lasp1 was confirmed, and MO specificity was validated through mRNA rescue experiments.
Conclusions:
- Lasp1 plays a significant role in zebrafish embryonic development and organogenesis.
- Zebrafish serve as a suitable model for studying Lasp1 function in developmental processes.
- Further research into Lasp1's developmental functions is warranted.
