Pathogenicity of PKCγ Genetic Variants-Possible Function as a Non-Invasive Diagnostic Biomarker in Ovarian Cancer

Area of Science:

• Oncology
• Genetics
• Molecular Biology

Background:

• Ovarian cancer, a leading gynecologic malignancy, suffers from high mortality due to late diagnosis.
• Genetic determinants are crucial for improving ovarian cancer outcomes.
• Protein Kinase C-γ (PKCγ) dysregulation is implicated in various cancers, with its variant rs1331262028 previously associated with hepatocellular carcinoma.

Purpose of the Study:

• To investigate the association between the PKCγ variant rs1331262028 and ovarian cancer.
• To determine the impact of this variant on PKCγ's protein interactions and molecular signaling.

Main Methods:

• Genotyping PCR was performed on a cohort of 100 participants to assess variant association.
• Protein-protein docking and molecular dynamic simulations were employed to analyze the variant's effect on PKCγ interactions.

Main Results:

• A significant positive association was found between the PKCγ variant rs1331262028 and ovarian cancer, including its clinicopathological features.
• Molecular dynamics simulations revealed the potential influence of the variant on PKCγ molecular signaling pathways.

Conclusions:

• The PKCγ variant rs1331262028 shows promise as a potential prognostic marker for ovarian cancer.
• Further clinical validation of this genetic variant could enable its application in patient management and diagnosis.