[⁶⁸Ga]Ga-NODAGA-E[(cRGDyK)]₂ and [⁶⁴Cu]Cu-DOTATATE PET Predict Improvement in Ischemic Cardiomyopathy

An increasing number of patients are living with chronic ischemic cardiomyopathy (ICM) and/or heart failure. Treatment options and prognostic tools are lacking for many of these patients. Our aim was to investigate the prognostic value of imaging angiogenesis and macrophage activation via positron emission tomography (PET) in terms of functional improvement after cell therapy. Myocardial infarction was induced in rats. Animals were scanned with [¹⁸F]FDG PET and echocardiography after four weeks and randomized to allogeneic adipose tissue-derived stromal cells (ASCs, n = 18) or saline (n = 9). Angiogenesis and macrophage activation were assessed before and after treatment by [⁶⁸Ga]Ga-RGD and [⁶⁴Cu]Cu-DOTATATE. There was no overall effect of the treatment. Rats that improved left ventricular ejection fraction (LVEF) had higher uptake of both [⁶⁸Ga]Ga-RGD and [⁶⁴Cu]Cu-DOTATATE at follow-up (p = 0.006 and p = 0.008, respectively). The uptake of the two tracers correlated with each other (r = 0.683, p = 0.003 pre-treatment and r = 0.666, p = 0.004 post-treatment). SUVmax at follow-up could predict improvement in LVEF (p = 0.016 for [⁶⁸Ga]Ga-RGD and p = 0.045 for [⁶⁴Cu]Cu-DOTATATE). High uptake of [⁶⁸Ga]Ga-RGD and [⁶⁴Cu]Cu-DOTATATE PET after injection of ASCs or saline preceded improvement in LVEF. The use of these tracers could improve the monitoring of heart failure patients in treatment.