Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Minor components of Hb Bart's.

E C Abraham1, A Abraham, J Kasten-Jolly

  • 1Department of Cell and Molecular Biology, Medical College of Georgia, Augusta 30912-3331.

Hemoglobin
|January 1, 1987
PubMed
Summary

Minor components of Hemoglobin Bart's (Hb Bart's) were analyzed, revealing the presence of acetylated and glycated forms in cord blood. This suggests gamma-chain glycation occurs readily in vivo, similar to major hemoglobin components.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

3D - pored Ixora Coccinea Linn. extracted nanocellulose-curcumin composites for tissue engineering applications.

International journal of biological macromolecules·2025
Same author

Monitoring the photosynthetic activity at single-cell level in <i>Haematococcus lacustris</i>.

Photosynthetica·2024
Same author

Single-cell microfluidics in combination with chlorophyll <i>a</i> fluorescence measurements to assess the lifetime of the <i>Chlamydomonas</i> PSBO protein.

Photosynthetica·2024
Same author

Assessing the impact of climate change on reproductive health: A content analysis of climate journalism coverage.

African journal of reproductive health·2024
Same author

Expanded carrier screening for inherited genetic disease using exome and genome sequencing.

Journal of genetic counseling·2024
Same author

Clinical efficacy of the virtual fracture clinic: analysis of 17,269 referrals by type of injury.

Annals of the Royal College of Surgeons of England·2022

Area of Science:

  • Biochemistry
  • Hematology
  • Analytical Chemistry

Background:

  • Hemoglobin Bart's (Hb Bart's) is a tetramer of gamma-globin chains, typically found in newborns.
  • Understanding the post-translational modifications of Hb Bart's is crucial for diagnosing and managing related hemoglobinopathies.

Purpose of the Study:

  • To characterize the minor components of Hb Bart's.
  • To investigate the presence and implications of acetylation and glycation in Hb Bart's.

Main Methods:

  • Separation of Hb Bart's minor components using CM-cellulose and DEAE-cellulose chromatography, and reverse-phase HPLC.
  • Characterization via amino acid analysis, tryptic peptide mapping (HPLC), electrophoresis, and carbohydrate/phosphate analysis.

Main Results:

  • Acetylated and glycated Hb Bart's components were identified in cord blood red cells.
  • The ratio of acetylated gamma chains (gamma Ic/total gamma) in Hb Bart's was similar to Hb F (fetal hemoglobin) components (Hb FIc/Hb F).
  • In vivo glycation of free gamma-chains appears to occur as readily as in major hemoglobin components.

Conclusions:

  • The gamma chain is not preferentially acetylated post-ribosomally compared to fetal hemoglobin.
  • Glycation of free gamma-chains in vivo is a significant post-translational modification, comparable to major hemoglobin components.

Related Experiment Videos