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Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
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In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...
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Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
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Growth media provide essential nutrients that support cell growth and metabolism, thereby enhancing the yield of valuable products such as enzymes, antibiotics, and biomass. Designing an effective growth medium involves balancing all components to prevent nutrient limitations or toxic excesses, both of which can impair growth and reduce product yields.Composition of a Typical Growth MediumA typical growth medium contains carbon and nitrogen sources, salts, vitamins, trace elements, and...
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Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
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Research on Biogas Yield from Macroalgae with Inoculants at Different Organic Loading Rates in a Three-Stage

Alvydas Zagorskis1, Regimantas Dauknys2, Mantas Pranskevičius1

  • 1Research Institute of Environmental Protection, Vilnius Gediminas Technical University, 10223 Vilnius, Lithuania.

International Journal of Environmental Research and Public Health
|January 21, 2023
PubMed
Summary

Macroalgae digestion in a three-stage bioreactor (TSB) yields optimal biogas production at a low organic loading rate (OLR) of 2.87 Kg VS/m³ d. This finding enhances the energy potential of biomass for a sustainable future.

Keywords:
anaerobic digestionbiogas yieldmacroalgaeorganic load ratethree-stage bioreactor

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Area of Science:

  • Biotechnology
  • Renewable Energy
  • Environmental Science

Background:

  • Macroalgae presents a sustainable alternative to fossil fuels.
  • Biogas production from macroalgae can significantly mitigate climate change.
  • Co-digestion with cattle manure and sewage sludge enhances biogas quality.

Purpose of the Study:

  • To investigate the biogas yield from Cladophora glomerata macroalgae using a three-stage bioreactor (TSB).
  • To determine the optimal organic loading rate (OLR) for maximizing biogas and methane yields.
  • To assess the biomass energy potential at different OLRs.

Main Methods:

  • Anaerobic digestion of Cladophora glomerata in a TSB with cattle manure and sewage sludge inoculants.
  • Testing of three organic loading rates: 2.87, 4.06, and 8.13 Kg VS/m³ d.
  • Measurement of biogas yield, methane yield, methane concentration, and biomass energy potential.

Main Results:

  • The highest biogas yield (439.0 ± 4.0 L/Kg VSadded) and methane yield (306.5 ± 9.2 L CH₄/Kg VSadded) were achieved at an OLR of 2.87 Kg VS/m³ d.
  • Increasing OLR to 4.06 and 8.13 Kg VS/m³ d resulted in a 1.55-fold decrease in biogas and methane yields.
  • Optimal decomposition of elements (C, N, H, S) occurred at 2.87 Kg VS/m³ d, leading to higher yields.
  • Methane concentration consistently remained high (68%-80%) across tested OLRs.
  • The maximum biomass energy potential was 3.05 kWh/Kg VSadded at the optimal OLR of 2.87 Kg VS/m³ d.

Conclusions:

  • An OLR of 2.87 Kg VS/m³ d is optimal for maximizing biogas and methane production from Cladophora glomerata in a TSB.
  • Optimizing OLR is crucial for enhancing the energy potential of macroalgae biomass.
  • This research supports the use of macroalgae as a viable feedstock for sustainable biogas production.