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An Automated High-Throughput Screening (HTS) Spotter for 3D Tumor Spheroid Formation.

Mi-Hyeon Jeong1, Inhee Kim1, Kyunghyun Park1

  • 1Central R & D Center, Medical & Bio Decision (MBD) Co., Ltd., Suwon-si 16229, Gyeonggi-do, Republic of Korea.

International Journal of Molecular Sciences
|January 21, 2023
PubMed
Summary

A new automated dispenser, the ASFA SPOTTER, precisely distributes cells in hydrogels for 3D organoid models. This technology enhances high-throughput screening (HTS) with speed, accuracy, and minimal sample waste.

Keywords:
3D spheroids/organoidsbioprinting-based dispenserhigh-throughput screening (HTS)miniaturization

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Area of Science:

  • Biotechnology
  • Biomedical Engineering
  • Cell Biology

Background:

  • Three-dimensional (3D) culture platforms are crucial for mimicking in vivo environments in high-throughput screening (HTS).
  • Existing automated dispensers face challenges in precisely dispensing cells within viscous hydrogels like Alginate and Matrigel.
  • Accurate and efficient cell dispensing is vital for developing robust 3D spheroid and organoid models.

Purpose of the Study:

  • To develop and refine an automated, contact-free dispensing machine (ASFA SPOTTER V5 and V6) for 3D cell culture applications.
  • To enable high-speed, low-volume dispensing of both viscous and non-viscous biosamples, including cells in hydrogels.
  • To improve the precision and accuracy of cell encapsulation for 3D spheroid and organoid model generation in HTS.

Main Methods:

  • Development of the ASFA SPOTTER (V5 and V6) automated dispensing machine.
  • Utilizing bioprinting techniques for dispensing cells within hydrogel scaffolds.
  • Testing compatibility with 96- and 384-well plates and micropillar/well chips.
  • Evaluating dispensing of non-viscous and viscous biosamples, including chemical drugs and cancer cells.

Main Results:

  • The ASFA SPOTTER enables precise, contact-free dispensing of cells in viscous hydrogels (over 70% viscosity) with minimal cross-contamination.
  • Dispensing volumes range from 20 to 4000 nanoliters, ensuring cell viability and integrity.
  • SPOTTER V6 offers an advantage of no washing steps and near-zero dead volume, enhancing efficiency.
  • Successful distribution of standard-sized cell spots with hydrogels onto 384-well plates was achieved with high speed, accuracy, and precision.

Conclusions:

  • The ASFA SPOTTER (V5 and V6) significantly advances automated cell dispensing for 3D spheroid and organoid cultures.
  • This technology facilitates miniaturization and automation for large-scale, high-throughput screening.
  • The machine's precision, speed, and minimal sample requirement make it ideal for developing advanced 3D cell-based assays.