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Clostridium butyricum Can Promote Bone Development by Regulating Lymphocyte Function in Layer Pullets.

Mengze Song1,2, Xuesong Zhang1,2, Guijuan Hao1,2

  • 1Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.

International Journal of Molecular Sciences
|January 21, 2023
PubMed
Summary

Supplementing laying hens with Clostridium butyricum (CB) enhances tibia development and bone formation markers. This probiotic also improves immune homeostasis by modulating cytokine expression and T regulatory cell ratios.

Keywords:
Clostridium butyricumTregboneimmune systemlymphocytesosteoblasts

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Area of Science:

  • Animal Science
  • Microbiology
  • Immunology

Background:

  • Bone health issues pose significant risks to laying hens.
  • Microbiome-based therapies offer a safe and cost-effective approach to managing bone health problems.

Purpose of the Study:

  • To investigate the effects of Clostridium butyricum (CB) supplementation on bone and immune homeostasis in pullets.
  • To elucidate the mechanisms by which CB influences bone formation and immune responses.

Main Methods:

  • In vivo studies involving CB supplementation in pullet feed.
  • In vitro experiments using osteoblast and lymphocyte cultures.
  • Analysis of bone formation markers (alkaline phosphatase, RUNX2) and immune parameters (IL-10, Treg cells).

Main Results:

  • CB supplementation improved tibia development and upregulated bone formation markers (alkaline phosphatase, RUNX2).
  • CB treatment increased IL-10 expression and the proportion of T regulatory cells in spleen and peripheral blood.
  • In vitro studies indicated that CB promotes osteoblast bone formation indirectly through lymphocyte mediation.

Conclusions:

  • Clostridium butyricum supplementation enhances bone health in laying pullets by modulating immune system cytokines and T regulatory cell ratios.
  • CB influences bone formation in laying hens via lymphocyte-mediated mechanisms.