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Playing with Biophysics: How a Symphony of Different Electromagnetic Fields Acts to Reduce the Inflammation in
Federica Zanotti1, Martina Trentini1, Ilaria Zanolla2
1Translational Medicine Department, University of Ferrara, 44121 Ferrara, Italy.
Abstract:
Several factors, such as ischemia, infection and skin injury impair the wound healing process. One common pathway in all these processes is related to the reactive oxygen species (ROS), whose production plays a vital role in wound healing. In this view, several strategies have been developed to stimulate the activation of the antioxidative system, thereby reducing the damage related to oxidative stress and improving wound healing. For this purpose, complex magnetic fields (CMFs) are used in this work on fibroblast and monocyte cultures derived from diabetic patients in order to evaluate their influence on the ROS production and related wound healing properties. Biocompatibility, cytotoxicity, mitochondrial ROS production and gene expression have been evaluated. The results confirm the complete biocompatibility of the treatment and the lack of side effects on cell physiology following the ISO standard indication. Moreover, the results confirm that the CMF treatment induced a reduction in the ROS production, an increase in the macrophage M2 anti-inflammatory phenotype through the activation of miRNA 5591, a reduction in inflammatory cytokines, such as interleukin-1 (IL-1) and IL-6, an increase in anti-inflammatory ones, such as IL-10 and IL-12 and an increase in the markers related to improved wound healing such as collagen type I and integrins. In conclusion, our findings encourage the use of CMFs for the treatment of diabetic foot.
Insights
Complex magnetic fields (CMFs) effectively promote wound healing in diabetic patients by reducing oxidative stress and inflammation. This innovative treatment enhances cell repair mechanisms, offering a promising therapeutic approach for diabetic foot ulcers.
Area of Science:
- Biomedical Engineering
- Cell Biology
- Wound Healing Research
Background:
- Wound healing is impaired by factors like ischemia, infection, and injury, often involving reactive oxygen species (ROS).
- Oxidative stress significantly damages cells, hindering the natural healing process.
- Diabetic patients exhibit particularly poor wound healing due to underlying metabolic and cellular dysfunctions.
Purpose of the Study:
- To investigate the effects of complex magnetic fields (CMFs) on ROS production and wound healing properties in diabetic patient-derived cells.
- To evaluate the biocompatibility and safety of CMF treatment.
- To explore CMFs' potential as a therapeutic strategy for diabetic wound complications.
Main Methods:
- Fibroblast and monocyte cultures from diabetic patients were exposed to CMFs.
- Assessed biocompatibility, cytotoxicity, mitochondrial ROS production, and gene expression.
- Analyzed inflammatory cytokine profiles and wound healing markers (collagen type I, integrins).
Main Results:
- CMF treatment demonstrated complete biocompatibility and no cytotoxicity, adhering to ISO standards.
- Reduced mitochondrial ROS production and inflammatory cytokines (IL-1, IL-6).
- Increased anti-inflammatory cytokines (IL-10, IL-12), M2 macrophage phenotype via miRNA 5591, and wound healing markers (collagen I, integrins).
Conclusions:
- CMFs are biocompatible and safe for treating diabetic cells.
- CMF application effectively modulates cellular responses to promote wound healing.
- CMFs show significant potential for treating diabetic foot ulcers by enhancing the antioxidative system and reducing inflammation.
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