Dysbiotic Gut Microbiota Modulation by Aronia Fruits Extract Administration

Area of Science:

• Microbiology
• Nutritional Science
• Biochemistry

Background:

• Long-term sweetener consumption, like stevia, can lead to gut dysbiosis.
• Chokeberry (Aronia) extract is investigated for its potential health benefits.
• Understanding the impact of natural extracts on gut microbiota is crucial.

Purpose of the Study:

• To evaluate chokeberry extract's capacity to modulate gut dysbiosis induced by stevia in an in vitro GIS1 system.
• To analyze the effects on microbial patterns, metabolomics, antioxidant status, and cytotoxicity.
• To identify potential biomarkers associated with chokeberry extract administration.

Main Methods:

• In vitro study using the GIS1 system.
• Molecular methods for microbial pattern determination.
• Metabolomic analysis (fatty acids) and antioxidant status (DPPH scavenging activity) assessment.
• Cytotoxicity evaluation over six months.

Main Results:

• Chokeberry extract demonstrated a strong antimicrobial effect, reducing Enterobacteriaceae and increasing favorable strains by 0.2 log UFC/mL.
• A transient increase in antioxidant status was observed, followed by a decrease correlated with microbial load reduction.
• Specific organic acids, including acetic and lactic acid, were present, with butyric acid remaining stable.
• The extract exhibited antimicrobial and cytotoxic effects, helping to correct dysbiosis.

Conclusions:

• Chokeberry extract effectively modulates stevia-induced gut dysbiosis in vitro.
• The extract possesses antimicrobial properties and influences gut metabolomics and antioxidant status.
• New organic acids in chokeberry extract may serve as valuable biomarkers for gut health modulation.