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The GSTO2 (rs156697) Polymorphism Modifies Diabetic Nephropathy Risk
Dragana Pavlovic1, Sinisa Ristic1, Ljubica Djukanovic2
1Faculty of Medicine, University of East Sarajevo, 73300 Foca, Bosnia and Herzegovina.
Glutathione transferase (GST) gene variations influence the risk of developing type 2 diabetes and diabetic nephropathy. These genetic factors also affect levels of advanced glycation end products (AGEs), impacting redox balance.
Area of Science:
- Genetics
- Endocrinology
- Biochemistry
Background:
- Type 2 diabetes mellitus (T2DM) development and complications involve genetic and environmental factors.
- Diabetic nephropathy (DN) is a major complication of T2DM, increasing cardiovascular risk and mortality.
- Glutathione transferases (GSTs) are crucial in detoxification and antioxidant defense.
Purpose of the Study:
- To investigate the modifying effect of glutathione transferase polymorphisms (GSTM1, GSTT1, GSTP1, GSTO1, GSTO2) on susceptibility to T2DM and DN.
- To assess the association between GST genotypes and advanced glycation end products (AGEs) levels in T2DM patients.
Main Methods:
- Genotyping of GSTM1, GSTT1, GSTP1, GSTO1, and GSTO2 polymorphisms using multiplex and real-time PCR.
- Analysis of 160 T2DM patients and 248 controls.
- Quantification of AGEs using ELISA.
Main Results:
- Significant associations were found between GSTM1, GSTT1, GSTP1 (rs1138272), and GSTO1 polymorphisms and T2DM susceptibility.
- The GSTO2 rs156697 polymorphism showed a significant association with DN in T2DM patients.
- Carriers of GSTM1 null, GSTT1 null, and variant GSTO1*AA genotypes exhibited significantly higher AGEs levels.
Conclusions:
- GST polymorphisms play a role in modulating the risk of T2DM and DN.
- These genetic variations influence AGEs concentration, suggesting a role in redox homeostasis.
- GST enzymes may be potential targets for managing diabetes complications.
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