Related Experiment Video
Updated: Aug 13, 2025

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography
Published on: April 16, 2021
Features of Mycobacterium bovis Complete Genomes Belonging to 5 Different Lineages
Ciriac Charles1,2, Cyril Conde2, Fabien Vorimore3
1Animal Health Laboratory, National Reference Laboratory for Tuberculosis, Paris-Est University, French Agency for Food, Environmental and Occupational Health and Safety (ANSES), CEDEX, 94701 Maisons-Alfort, France.
Abstract:
Mammalian tuberculosis (TB) is a zoonotic disease mainly due to Mycobacterium bovis (M. bovis). A current challenge for its eradication is understanding its transmission within multi-host systems. Improvements in long-read sequencing technologies have made it possible to obtain complete bacterial genomes that provide a comprehensive view of species-specific genomic features. In the context of TB, new genomic references based on complete genomes genetically close to field strains are also essential to perform precise field molecular epidemiological studies. A total of 10 M. bovis strains representing each genetic lineage identified in France and in other countries were selected for performing complete assembly of their genomes. Pangenome analysis revealed a "closed" pangenome composed of 3900 core genes and only 96 accessory genes. Whole genomes-based alignment using progressive Mauve showed remarkable conservation of the genomic synteny except that the genomes have a variable number of copies of IS6110. Characteristic genomic traits of each lineage were identified through the discovery of specific indels. Altogether, these results provide new genetic features that improve the description of M. bovis lineages. The availability of new complete representative genomes of M. bovis will be useful to epidemiological studies and better understand the transmission of this clonal-evolving pathogen.
More Related Videos
08:03Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
Published on: December 7, 2021
11:09Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment Kartchner Caverns, AZ, USA
Published on: January 2, 2015
Related Concept Videos
Modern Molecular Taxonomy
Bacterial Phylum Actinobacteria
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
Evolutionary Relationships through Genome Comparisons
Bacterial Phylum Tenericutes
Genomic DNA in Prokaryotes
Genomic Diversity in Bacteria
Although bacterial genomes are much...