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Updated: Aug 13, 2025

Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control
Published on: April 11, 2013
Epiphytic and Endophytic Fungi Colonizing Seeds of Two Poaceae Weed Species and Fusarium spp. Seed Degradation
Jevgenija Ņečajeva1, Anete Borodušķe1,2, Vizma Nikolajeva1,2
1Institute of Plant Protection Research 'Agrihorts', Latvia University of Life Sciences and Technologies, Paula Lejiņa Iela 2, LV-3004 Jelgava, Latvia.
Abstract:
Fungi colonizing the surface and endosphere of two widespread Poaceae weed species, Avena fatua and Echinochloa crus-galli, were isolated to compare the taxonomic composition between the plant species, location, and year of the seed collection. The seed-degrading potential of Fusarium isolated from the seeds was tested by inoculating seeds of E. crus-galli with spore suspension. Molecular identification of epiphytic and endophytic fungal genera was performed by sequencing the ITS region of rDNA. Endophytes comprised of significantly lower fungal richness compared to epiphytes. A significant taxonomic overlap was observed between the endosphere and seed surface. The most abundant genera were Alternaria, Fusarium, Cladosporium, and Sarocladium. Analysis of similarities and hierarchical clustering showed that microbial communities were more dissimilar between the two plant species than between the years. Fusarium isolates with a high potential to infect and degrade E. crus-galli seeds in laboratory conditions belong to F. sporotrichioides and F. culmorum.
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