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Use of biotinylated probes in serum hepatitis B virus DNA detection
J A Saldanha1, P Karayiannis, H C Thomas
1Department of Physiology, Royal Free Hospital School of Medicine, London, U.K.
Journal of Virological Methods
|July 1, 1987
Abstract:
A dot-blot hybridisation assay for serum HBV DNA is described using a non-radioactive (biotinylated) DNA probe. The assay is both sensitive (1 pg of HBV DNA) and reproducible, and shows several advantages over similar assays which use 32P-labelled probes for the routine detection of infectious virus particles both in blood and in other biological fluids.