Biocompatible Silica-Polyethylene Glycol-Based Composites for Immobilization of Microbial Cells by Sol-Gel Synthesis

Biocatalysts based on the methylotrophic yeast Ogataea polymorpha VKM Y-2559 immobilized in polymer-based nanocomposites for the treatment of methanol-containing wastewater were developed. The organosilica composites with different matrix-to-filler ratios derived from TEOS/MTES in the presence of PEG (SP_EG-composite) and from silicon-polyethylene glycol (STP_EG-composite) differ in the structure of the silicate phase and its distribution in the composite matrix. Methods of fluorescent and scanning microscopy first confirmed the formation of an organosilica shell around living yeast cells during sol-gel bio-STP_EG-composite synthesis. Biosensors based on the yeast cells immobilized in STP_EG- and SP_EG-composites are characterized by effective operation: the coefficient of sensitivity is 0.85 ± 0.07 mgO₂ × min^-1 × mmol^-1 and 0.87 ± 0.05 mgO₂ × min^-1 × mmol^-1, and the long-term stability is 10 and 15 days, respectively. The encapsulated microbial cells are protected from UV radiation and the toxic action of heavy metal ions. Biofilters based on the developed biocatalysts are characterized by high effectiveness in the utilization of methanol-rich wastewater-their oxidative power reached 900 gO₂/(m³ × cycle), and their purification degree was up to 60%.