Related Experiment Video
Updated: Aug 13, 2025
08:57
Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
Published on: July 17, 2021
7.5K
Advances in CRISPR-Cas9 for the Baculovirus Vector System: A Systematic Review
Duygu Sari-Ak1, Omar Alomari2, Raghad Al Shomali2
1Department of Medical Biology, Hamidiye International School of Medicine, University of Health Sciences, 34668 Istanbul, Turkey.
Viruses
|January 21, 2023
Summary
The BacMam system enables protein expression in mammalian cells, evolving from baculovirus expression vector systems. This review explores its integration with CRISPR-Cas technologies for genome editing and glycoengineering, advancing protein production and gene therapy.
Area of Science:
- Biotechnology
- Molecular Biology
- Genomics
Background:
- Baculovirus expression vector systems (BEVS) are effective for recombinant protein production in insect cells.
- Baculovirus does not replicate in mammalian cells, necessitating alternative systems.
- The BacMam system was developed to enable transgene expression in mammalian cells, with MultiBacMam allowing multi-protein expression.
Conclusions:
- The continued development of BEVS, particularly BacMam, enhances protein expression capabilities in mammalian systems.
- CRISPR-Cas integration with BEVS offers powerful tools for genome editing and glycoengineering.
- CRISPR-Cas9 is poised to significantly impact protein expression, gene therapy, and genome engineering.
Related Concept Videos
01:28CRISPR/Cas9 Genome Editing
119
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
119
01:59CRISPR
52.6K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.6K
02:53CRISPR and crRNAs
17.2K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.2K
01:23The Antiviral System of Bacteria and Archaea: CRISPR
84
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
84