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Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay
Published on: October 4, 2024
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Pathways Activated by Infected and Bystander Chondrocytes in Response to Ross River Virus Infection
Elisa X Y Lim1, Julie A Webster1, Penny A Rudd1
1Institute for Glycomics, Gold Coast Campus, Griffith University, Southport, QLD 4222, Australia.
Viruses
|January 21, 2023
Summary
Ross River virus (RRV) infection affects cartilage cells. RNA-sequencing revealed distinct gene expression in infected and bystander chondrocytes, highlighting the need for targeted therapies to protect healthy cells.
Area of Science:
- Virology
- Molecular Biology
- Immunology
Background:
- Old World alphaviruses, like Ross River virus (RRV), cause joint pain (arthralgia) due to cartilage degradation.
- Chondrocytes, key cartilage cells, are crucial for matrix maintenance and may play a role in RRV pathogenesis.
Purpose of the Study:
- To investigate the transcriptomic differences between RRV-infected and bystander chondrocytes.
- To identify unique cellular responses in both infected and uninfected cells within the same environment.
Main Methods:
- RNA-sequencing (RNA-Seq) was employed to analyze gene expression profiles.
- Green fluorescent protein (GFP) tagging of RRV enabled the isolation of infected (GFP+) and bystander (GFP-) chondrocytes.
Main Results:
- While infected and bystander chondrocytes shared some gene expression profiles, unique signatures were observed.
- RIMS2 and FOXJ1 were uniquely expressed in infected cells.
- Aim2 and CCL8 were uniquely expressed in bystander cells.
Conclusions:
- Understanding differential gene expression is crucial for developing targeted RRV therapies.
- Therapeutic strategies must consider potential adverse effects on neighboring uninfected chondrocytes.
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