Dissecting contributions of representative heavy metal components in PM_2.5 to its cytotoxicity

PM_2.5 is a complex pollutant that is a pervasive threat to human health. The health risks and toxicity mechanisms of PM_2.5 components must be identified to alleviate the corresponding risks. In this study, a reductionism approach based on model PM_2.5 particles was used to investigate the contributions of the most harmful components in PM_2.5 to its toxicity. Human liver and kidney cells were used as models. The results showed that Cr(VI) was the most critical toxic component among other components (Pb, As, and benzo[a]pyrene) in human liver and kidney cells. PM_2.5-Cr(VI) induced oxidative stress, which led to cytotoxicity by inducing cell cycle arrest in the S-phase in HepG2 and HEK293 cells. The presented findings can provide valuable insights into the toxicity levels of PM_2.5 components, which can help clarify the potential health risks from PM_2.5 exposure.