An achromatic colorimetric nanosensor for sensitive multiple pathogen detection by coupling plasmonic nanoparticles...

Area of Science:

• Nanotechnology
• Biosensing
• Pathogen Detection

Background:

• Rapid screening of multiple pathogens is crucial for pandemic prevention and control.
• Existing colorimetric methods offer convenience but struggle with simultaneous detection, low visual resolution, and sensitivity.
• Need for advanced diagnostic tools for efficient food safety and quality monitoring.

Purpose of the Study:

• To develop an achromatic colorimetric nanosensor for simultaneous detection of SARS-CoV-2, Staphylococcus aureus, and Salmonella typhimurium.
• To enhance visual resolution and sensitivity in pathogen detection compared to traditional colorimetric sensors.
• To provide a convenient and efficient tool for food safety monitoring and early warning systems.

Main Methods:

• Coupling three types of plasmonic nanoparticles (gold and silver) with magnetic separation.
• Utilizing specific nanoparticle-pathogen recognition for sandwich complex formation.
• Employing magnetic separation to isolate target complexes and induce distinct color changes.

Main Results:

• Developed an achromatic nanosensor that changes from black to specific chromatic colors upon detection of individual or multiple pathogens.
• Achieved simultaneous detection of SARS-CoV-2, S. aureus, and S. typhimurium with enhanced visual resolution and sensitivity.
• Demonstrated distinct color responses for single and mixed pathogen infections (e.g., green for SARS-CoV-2, purple for S. aureus, orange for S. typhimurium).

Conclusions:

• The achromatic nanosensor enables convenient and highly sensitive simultaneous pathogen detection.
• This sensor offers a promising approach for efficient monitoring and early warning in food safety and quality control.
• The developed technology significantly improves upon existing colorimetric methods for pathogen screening.