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Updated: Aug 13, 2025

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
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TNT version 1.6, with a graphical interface for MacOS and Linux, including new routines in parallel.

Pablo A Goloboff1,2, Martín E Morales1

  • 1Unidad Ejecutora Lillo (Consejo Nacional de Investigaciones Científicas y Técnicas - Fundación Miguel Lillo), Miguel Lillo 251, 4000 S.M. de Tucumán, Argentina.

Cladistics : the International Journal of the Willi Hennig Society
|January 22, 2023
PubMed
Summary

A new graphical user interface (GUI) for TNT phylogenetic analysis software is now available for Linux and Mac. This enhanced TNT version 1.6 offers improved usability, parallel processing, and 64-bit compatibility for large datasets.

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Area of Science:

  • Computational Biology
  • Bioinformatics
  • Phylogenetics

Background:

  • The TNT (The Next Generation) program is a widely used software for phylogenetic analysis.
  • Previous versions primarily offered a graphical user interface (GUI) exclusively for Windows operating systems.
  • Advancements in computational biology necessitate more accessible and efficient phylogenetic tools across diverse operating systems.

Purpose of the Study:

  • To introduce a new, cross-platform graphical user interface (GUI) for the TNT phylogenetic analysis software.
  • To enhance the functionality and user experience of the TNT program for a broader range of operating systems.
  • To detail the improvements and new features introduced in TNT version 1.6.

Main Methods:

  • Development of a new GUI based on the GIMP Tool Kit (GTK version 3).
  • Implementation of cross-platform compatibility for Linux, macOS, and Windows (via Cygwin).
  • Compilation of binaries as native 64-bit applications to overcome memory limitations.

Main Results:

  • The new GUI provides access to dialogs via simple commands and enables direct saving of tree diagrams in Scalable Vector Graphics (SVG) format.
  • The updated version supports parallel analysis management using the Parallel Virtual Machine (PVM) system.
  • Native 64-bit compilation removes memory access limitations present in the previous 32-bit Windows GUI.

Conclusions:

  • The release of TNT version 1.6 with its new GTK-based GUI significantly improves accessibility and functionality for phylogenetic analyses on multiple operating systems.
  • Enhanced features such as SVG export and parallel processing capabilities streamline complex phylogenetic workflows.
  • The transition to 64-bit native applications ensures better performance and scalability for handling large biological datasets.