Measurement of the alpha 2 component of a novel oncodevelopmental antigen by a rapid electroimmunoassay

A method was required for the quantification of a novel oncodevelopmental antigen which increases in the serum of pregnant women as well as patients with ovarian cancer. The native antigen is composed of two components having alpha 2 and beta electrophoretic mobilities. An assay method, involving electroimmunodiffusion according to Laurell, was developed for the measurement of the alpha 2 component of the antigen. The method is sufficiently sensitive to determine significant differences in the levels of antigen in the serum of non-pregnant and pregnant subjects, and patients with ovarian cancer. In addition to routine quantitation, the technique will also be an indispensable analytical tool in the purification of the protein.