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Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
Published on: August 8, 2016
The complete genome sequence of Pseudomonas chengduensis BC1815 for genome mining of PET degrading enzymes
Zhengguang Shi1, Xue Yu1, Jingjing Duan2
1School of Advanced Manufacturing, Fuzhou University, Jinjiang 362251, China; Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, Fujian, China.
Abstract:
Pseudomonas chengduensis BC1815, isolated from a marine sediment sample of the Pacific Ocean, can grow in mineral medium with PET plastic as sole carbon source. Here, we present the complete genome of Pseudomonas chengduensis BC1815, which will facilitate the genome mining of PET degrading enzymes. The total length of the sequenced genome consists of 5,578,440 bases, with mean G + C content of 62.65%. A total of 5150 coding genes including 65 tRNAs and 12 rRNAs were predicted in the genome. Thirteen proteins of esterase, lipase and α/β hydrolase were taken as candidates for PET degrading enzymes, in which eight were membrane bounded and the others were secretory.
