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Related Experiment Video

Updated: Aug 13, 2025

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods

Published on: December 23, 2013

Rapid prototyping for high-pressure microfluidics.

Carlie Rein¹, Mehmet Toner^1,2,3, Derin Sevenler^4,5,6

¹Center for Engineering in Medicine and Surgery, Massachusetts General Hospital, Boston, MA, USA.

Scientific Reports

|January 22, 2023

Summary

This summary is machine-generated.

This study presents a method for fabricating rigid microfluidic devices using epoxy and glass, overcoming the limitations of flexible polydimethylsiloxane (PDMS) for high-pressure applications like cell focusing.

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Area of Science:

Microfluidics
Materials Science
Biotechnology

Background:

Soft lithography using polydimethylsiloxane (PDMS) is common for microfluidic device fabrication.
PDMS flexibility limits its use in high-pressure or high-resolution microfluidics applications.
Rigid materials like epoxy and glass offer improved mechanical performance and feature resolution.

Purpose of the Study:

To provide a detailed fabrication method for rigid microfluidic devices using epoxy and glass.
To optimize a bonding protocol for enhanced device robustness.
To demonstrate the utility of these rigid devices in high-throughput cell focusing.

Main Methods:

Soft lithography was employed to pattern molds for epoxy and glass microfluidic devices.
A novel bonding protocol was developed and optimized for rigid materials.
High aspect ratio spiral microchannels were fabricated using the developed method.

Main Results:

Fabricated rigid microfluidic devices demonstrated superior mechanical performance compared to PDMS.
The optimized bonding protocol enabled devices to withstand pressures exceeding 500 psi.
High throughput cell focusing was successfully achieved using the rigid spiral microchannels.

Conclusions:

The presented method enables the reliable fabrication of rigid microfluidic devices from epoxy and glass.
These rigid devices are suitable for demanding microfluidic applications requiring high pressure and precision.
The technology facilitates advanced cell manipulation techniques, such as high-throughput cell focusing.