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Related Experiment Video

Updated: Aug 13, 2025

Intra-lymph Node Injection of Biodegradable Polymer Particles
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In vitro Function Study of Different Negative Charge Pullulan Nanoparticles for Sentinel Lymph Node Angiography.

Ren Feng Huang1, Yan Guo1, Chaoling Yao1

  • 1Department of Breast Surgery, Yue Bei People's Hospital, Shaoguan, China.

Current Drug Delivery
|January 23, 2023
PubMed
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Clinical breast cancer·2022

Researchers developed novel anionic pullulan nanoparticles (NPs) to improve sentinel lymph node (SLN) imaging. The CHPC2 nanoparticles demonstrated optimal negative surface charge and enhanced indocyanine green (ICG) stability, offering a promising alternative for SLN procedures.

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Medical Imaging

Background:

  • Traditional sentinel lymph node (SLN) dyes and radioactive markers have limitations including false positives and radiation injury.
  • Indocyanine green (ICG) offers lower false positive rates and tissue damage but lacks a clear operative field of vision.

Purpose of the Study:

  • To synthesize novel anionic pullulan nanoparticles (NPs) for improved SLN imaging.
  • To control nanoparticle size and surface potential for enhanced indocyanine green (ICG) delivery and visualization.

Main Methods:

  • Synthesized three anionic pullulan derivatives (CHP, CHPC1, CHPC2) with varying hydrophobic and succinyl degrees.
  • Characterized nanoparticle size (<50nm TEM, ~90-115nm hydrodynamic), zeta potential (-1.9 to -19.4 mV), and ICG loading.
Keywords:
CHPC1Indocyanine green (ICG)Radioactiveanionic pullulan materialsnanoparticle (NPs)sentinel lymph node

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  • Assessed nanoparticle toxicity to normal cells (HSF) and cellular uptake.

    • Main Results:

    • Nanoparticle size and negative surface potential increased with higher succinyl content.
    • ICG-loaded NPs showed increased size and negative zeta potential, with CHPC2 exhibiting the largest negative charge (-23.1 mV).
    • Higher succinyl content correlated with lower ICG release, increased ICG stability, and decreased cellular uptake; blank NPs showed no significant toxicity.

    Conclusions:

    • Successfully prepared CHPC2 carriers with maximum negative surface charge, suitable for further research.
    • Developed novel anionic pullulan nanoparticles as potential carriers for ICG in SLN procedures.
    • Provided new ideas for advancing SLN imaging techniques.