Caudatin attenuates inflammatory reaction by suppressing JNK/AP-1/NF-κB/caspase-1 pathways in activated HMC-1 cells

Area of Science:

• Immunology
• Pharmacology
• Natural Products

Background:

• Cytokine storm and mast cell activation contribute to COVID-19 hyperinflammation.
• Identifying natural compounds to modulate these inflammatory pathways is crucial.

Purpose of the Study:

• To investigate the anti-inflammatory effects of caudatin on human mast cell line HMC-1.
• To explore caudatin's potential in mitigating mast cell-mediated inflammation.

Main Methods:

• HMC-1 cells were treated with varying doses of caudatin.
• Key signaling molecules including JNK, AP-1, NF-κB, and caspase-1 were analyzed.
• mRNA expression and protein synthesis of pro-inflammatory cytokines were assessed.

Main Results:

• Caudatin suppressed JNK, AP-1, and NF-κB activation by inhibiting IκBα phosphorylation.
• Caudatin reduced activated-caspase-1 levels and inhibited its activation.
• Non-toxic doses of caudatin decreased pro-inflammatory cytokine mRNA and protein levels.

Conclusions:

• Caudatin effectively inhibits the JNK/AP-1/NF-κB/caspase-1 signaling pathway in mast cells.
• Caudatin reduces the secretion of inflammatory cytokines, offering potential therapeutic benefits.
• Caudatin may serve as a valuable ingredient in functional foods for inflammatory conditions like COVID-19.