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Protocol for UVC uridine actinometry
Dana Pousty1, Hadas Mamane1, Vered Cohen-Yaniv1
1School of Mechanical Engineering, Faculty of Engineering, Tel-Aviv University, Tel-Aviv, Israel.
Methodsx
|January 23, 2023
Summary
Uridine actinometry offers a simple, eco-friendly method for measuring germicidal UV light. This technique is ideal for validating the photon flux of UVC disinfection systems, including both mercury lamps and UV-LEDs.
Area of Science:
- Photochemistry
- Microbiology
- Environmental Science
Background:
- Uridine, containing the uracil chromophore, shares absorption spectra with DNA between 240-290 nm.
- This spectral overlap aligns with the inactivation sensitivity of most microorganisms to UV light.
- Uridine's absorption maximum at 262 nm makes it suitable for measuring germicidal photon flux.
Purpose of the Study:
- To present uridine actinometry as an ideal method for determining germicidal photon flux.
- To validate the photon flux of Ultraviolet C (UVC) disinfection systems.
- To offer a simple, environmentally friendly, and user-friendly actinometry protocol.
Main Methods:
- Uridine actinometry utilizes the absorbance spectrum of uridine to quantify UV light.
- The protocol is designed for accuracy and ease of operation.
- It is adaptable for various UVC sources, including low-pressure mercury lamps and UV-LEDs.
Main Results:
- Uridine actinometry is highly effective for validating the photon flux of UVC systems.
- The method is suitable for both conventional low-pressure mercury lamps and emerging UV-LED disinfection technologies.
- Initial uridine concentration and photoproduct absorbance influence kinetic order and quantum yield, allowing for protocol customization.
Conclusions:
- Uridine actinometry provides an accurate, simple, and environmentally sound approach for UVC system validation.
- Its applicability to both UV-LP and UV-LED sources makes it versatile for water disinfection.
- The protocol's customizable parameters ensure precise photon flux determination.
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