Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Folding Quality Check in the RER01:29

Protein Folding Quality Check in the RER

3.8K
ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
3.8K
Quality Assurance01:19

Quality Assurance

174
Quality assurance is the overarching term used to describe the activities employed to ensure the proper performance of a system. These activities can be classified into three categories: quality control, quality assessment, and internal corrective measures. Typically, these activities work cyclically: quality control is performed before and during the analysis, while quality assessment occurs during and after the investigation. Internal corrective measures are implemented based on the findings...
174
Proteins: Dietary Sources and Requirements01:28

Proteins: Dietary Sources and Requirements

544
Consuming animal-based products offers high-quality proteins that contain optimal levels and combinations of essential amino acids, crucial for tissue repair and growth. Foods like eggs, milk, fish, and most meats are a source of complete proteins. Legumes and cereals are abundant in proteins; however, they typically lack a full range of essential amino acids. As a result, they are considered incomplete protein sources. Some plant sources like soybeans, quinoa, and amaranth do contain complete...
544
Quality Control01:05

Quality Control

218
Quality control is one of the three cyclical quality assurance activities that help keep a system under statistical control. Typical quality control activities include creating quality control charts, conducting proficiency testing, and documenting and archiving results.
Quality control helps track data, visualize trends, and identify variations, making it easier to detect deviations that may affect the accuracy of an analysis. One way to do this is by generating a quality control chart, which...
218
Bioequivalence: Overview01:16

Bioequivalence: Overview

1.1K
Pharmaceutical equivalents, by definition, are drug products with the same active ingredient in the same quantities, encapsulated in identical dosage forms, and intended for the same administration routes. These pharmaceutical equivalents are deemed bioequivalent if the bioavailability of the active entity in the drug preparations is similar. Moreover, pharmaceutical equivalents demonstrating bioequivalence are also regarded as therapeutically equivalent. This means that when used as directed,...
1.1K
Key Elements for Plant Nutrition02:35

Key Elements for Plant Nutrition

18.9K
Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...
18.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Turbidity and biotracer evidence reveal reorganisation of sediment sources and delivery during wet winters with moderate rainfall events.

Environmental research·2026
Same author

Can large-scale climate patterns predict nitrate export mechanisms from agricultural land?

Chemosphere·2026
Same author

Advancing mechanistic understanding of cohesive sediment transport: Integrating flume experiments, field measurements, and modelling approaches in a gravel-bed river.

The Science of the total environment·2024
Same author

The role of ruminant urine and faeces in the recycling of nutrients by forages.

Scientific reports·2024
Same author

Do NO, N<sub>2</sub>O, N<sub>2</sub> and CO<sub>2</sub> fluxes differ in soils sourced from cropland and varying riparian buffer vegetation? An incubation study.

Soil use and management·2024
Same author

A commentary on key methodological developments related to nutritional life cycle assessment (nLCA) generated throughout a 6-year strategic scientific programme.

Food and energy security·2024

Related Experiment Video

Updated: Aug 13, 2025

Quantifying Plant Soluble Protein and Digestible Carbohydrate Content, Using Corn Zea mays As an Exemplar
07:19

Quantifying Plant Soluble Protein and Digestible Carbohydrate Content, Using Corn Zea mays As an Exemplar

Published on: August 6, 2018

20.4K

Protein quality as a complementary functional unit in life cycle assessment (LCA).

G A McAuliffe1, T Takahashi1,2, T Beal3,4

  • 1Net Zero and Resilient Farming, Rothamsted Research, North Wyke, Okehampton, EX20 2SB Devon UK.

The International Journal of Life Cycle Assessment
|January 23, 2023
PubMed
Summary

Nutritional life cycle assessments (nLCAs) should consider protein quality, not just quantity, for accurate dietary recommendations. Incorporating the Digestible Indispensable Amino Acid Score (DIAAS) improves environmental impact assessments of foods.

Keywords:
Amino acidsDigestibilityEnvironmental footprintsFoodHealthNutritionNutritional LCA

More Related Videos

Author Spotlight: Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality
05:45

Author Spotlight: Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality

Published on: April 7, 2023

3.5K
A Quantitative Fitness Analysis Workflow
11:39

A Quantitative Fitness Analysis Workflow

Published on: August 13, 2012

14.6K

Related Experiment Videos

Last Updated: Aug 13, 2025

Quantifying Plant Soluble Protein and Digestible Carbohydrate Content, Using Corn Zea mays As an Exemplar
07:19

Quantifying Plant Soluble Protein and Digestible Carbohydrate Content, Using Corn Zea mays As an Exemplar

Published on: August 6, 2018

20.4K
Author Spotlight: Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality
05:45

Author Spotlight: Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality

Published on: April 7, 2023

3.5K
A Quantitative Fitness Analysis Workflow
11:39

A Quantitative Fitness Analysis Workflow

Published on: August 13, 2012

14.6K

Area of Science:

  • Nutritional Life Cycle Assessment (nLCA)
  • Dietary environmental impact analysis
  • Protein quality and bioavailability

Background:

  • Life cycle assessment (LCA) studies often recommend restricting animal-sourced foods due to environmental impacts.
  • These studies may oversimplify nutritional considerations, using generic protein requirements instead of specific nutrient bioavailability.
  • Animal-sourced foods are crucial for nutrient adequacy in vulnerable global populations.

Purpose of the Study:

  • To evaluate the necessity of considering protein quality in nutritional LCA (nLCA) when using protein as a nutritional functional unit (nFU).
  • To explore the role of the Digestible Indispensable Amino Acid Score (DIAAS) in nLCA for correcting amino acid quality and digestibility.
  • To demonstrate the strengths and weaknesses of protein-quality adjustment through a case study.

Main Methods:

  • An exploratory virtual experiment was conducted to assess DIAAS in nLCA.
  • The study focused on single food items, analyzing 4 animal-based and 4 plant-based products.
  • A sensitivity analysis using beef explored how different cuts impact nLCA interpretation.

Main Results:

  • Protein-quality correction, particularly using DIAAS, is essential for accurate nLCA when protein is the nFU.
  • DIAAS quantifies the contribution of digestible indispensable amino acids relative to daily requirements for protein synthesis.
  • Considering protein complementarity at the meal-level, rather than just the product level, is crucial for assessing consumer metabolic responses.

Conclusions:

  • nLCA studies should incorporate protein quality, ideally quantitatively using metrics like DIAAS, as data availability increases.
  • Future research should prioritize meal-level amino acid balance analysis over product-level analysis.
  • Evaluating 'protein-rich foods' necessitates including a broader range of nutrients beyond just amino acids for comprehensive dietary assessment.